Spatial competition with Lactococcus lactis in mixed-species continuous-flow biofilms inhibits Listeria monocytogenes growth
Authored by Olivier Habimana, Laurent Guillier, Saulius Kulakauskas, Romain Briandet
Date Published: 2011
DOI: 10.1080/08927014.2011.626124
Sponsors:
No sponsors listed
Platforms:
MATLAB
Model Documentation:
Other Narrative
Model Code URLs:
Model code not found
Abstract
Surfaces in industrial settings provide a home for resident biofilms
that are likely to interact with the attachment, growth and survival of
pathogens such as Listeria monocytogenes. Experimental results have
indicated that L. monocytogenes cells were inhibited by the presence of
a model resident flora (Lactococcus lactis) in dual-species continuous
flow-biofilms, and are spatially restricted to the lower biofilm layers.
Using a new, simplified individual-based model (IBM) that simulates
bacterial cell growth in a three-dimensional space, the spatial
arrangements of the two species were reconstructed and their cell counts
successfully predicted. This model showed that the difference in
generation times between L. monocytogenes and L. lactis cells during the
initial stages of dual-species biofilm formation was probably
responsible for the species spatialization observed and the subsequent
inhibition of growth of the pathogen.
Tags
microorganisms
Variability
Pathogen
Food
Single cells
Microbiology
Smear cheese
Wooden shelves
Lag time
Temperatures