Bacteria can form interconnected microcolonies when a self-excreted product reduces their surface motility: evidence from individual-based model simulations

Authored by Guillaume Deffuant, Nabil Mabrouk, Tim Tolker-Nielsen, Claude Lobry

Date Published: 2010

DOI: 10.1007/s12064-009-0078-8

Sponsors: No sponsors listed

Platforms: No platforms listed

Model Documentation: ODD Mathematical description

Model Code URLs: Model code not found

Abstract

Recent experimental observations of Pseudomonas aeruginosa, a model bacterium in biofilm research, reveal that, under specific growth conditions, bacterial cells form patterns of interconnected microcolonies. In the present work, we use an individual-based model to assess the involvement of bacteria motility and self-produced extracellular substance in the formation of these patterns. In our simulations, the pattern of interconnected microcolonies appears only when bacteria motility is reduced by excreted extracellular macromolecules. Immotile bacteria form isolated microcolonies and constantly motile bacteria form flat biofilms. Based on experimental data and computer simulations, we suggest a mechanism that could be responsible for these interconnected microcolonies.
Tags
ecology growth Protocol Involvement Microbial biofilms Iv pili Multicellular structures Cellular-automaton approach Pseudomonas-aeruginosa biofilms Extracellular dna