Cell aggregation in monolayer culture: Clues to a universal kinetics
Authored by Silva P C A da, A A Santos, S G Alves, M L Martins
Date Published: 2018
DOI: 10.1016/j.physa.2018.07.035
Sponsors:
Brazilian Ministry of Education (CAPES)
Brazilian National Council for Scientific and Technological Development (CNPq)
Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)
Platforms:
No platforms listed
Model Documentation:
Other Narrative
Model Code URLs:
Model code not found
Abstract
Aggregation of animal cells in culture comprises a series of motility,
collision and adhesion processes of basic relevance for tissue
engineering, bioseparations, oncology research and in vitro drug
testing. In the present paper, we characterize the aggregation kinetics
of normal and cancer cells in culture by determining their cluster size
distribution functions. Our experiments reveal that the complementary
cumulative distributions for eight cell lines from epithelial and
mesenchymal origins are described by stretched exponential functions.
Also, we find that as time evolves and the cell density increases, a
dynamical phase transition occurs from an aggregation regime described
by monotonically decreasing cluster size distributions to another one in
which these distributions are nonmonotonic, exhibiting local maxima at
large cluster sizes. Subtly, the universality of the stretched
exponential distributions admits distinct cluster growth processes
controlled by motility and adhesion traits of the cells. (C) 2018
Elsevier B.V. All rights reserved.
Tags
cell culture
Dynamics
Distributions
Clusters
morphogenesis
Spheroids
Aggregation processes
Cluster size distributions
Hybrid
agent-based modeling
Prostate-cancer cells
Du-145